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具体情况如下：
Molecular cloning and characterization of pathogenesis-related protein 5 in Zea mays and its antifungal activity against Rhizoctonia solani
作者: Liu, L (Liu, Li)1; Zhang, ZM (Zhang, Zhiming)1; Zhao, MJ (Zhao, Maojun)2; Wang, J (Wang, Jing)1; Lin, HJ (Lin, Hai-jian)1; Shen, YO (Shen, Ya-ou)1; Pan, GT (Pan, Guangtang)1
来源出版物: AFRICAN JOURNAL OF BIOTECHNOLOGY 卷: 10 期: 83 页: 19286-19293 DOI: 10.5897/AJB11.696 出版年: DEC 21 2011
被引频次: 0 (来自 Web of Science)
引用的参考文献: 23 [ 查看 Related Records ] 引证关系图
摘要: We described the cloning and characterization of pathogenesis-related protein 5 gene in maize, named ZmPR5 (GenBank Accession Number: HM230665). Molecular and bioinformatic analyses of ZmPR5 revealed an open reading frame (ORF) of 525 bp, encoding a protein of 175 amino acids (aa) and a deduced molecular mass of 17.5 KDa. Homology analysis of the Zea mays L. deduced amino acid sequence, indicated homology between 40 and 74% with Oryza sativa, Hordeum vulgare, and Triticum aestivum, among others. Real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of ZmPR5 is constantly higher in the maize resistant inbred line R15 compared with that in the susceptible inbred line 478. Moreover, the ZmPR5 gene was up-regulated after it was challenged by Rhizoctonia solani. We subsequently purified the recombinant protein and analyzed its antimicrobial activities in vitro. The results obtained show that the recombinant protein inhibited hyphal growth of R. solani. This study suggests that the expression of ZmPR5 is closely related to maize sheath blight resistance caused by R. solani. Further, the antifungal activity of ZmPR5 showed that ZmPR5 plays an important role in the disease resistance response.
入藏号: WOS:000298561500005
文献类型: Article
语种: English
作者关键词: Maize; ZmPR5; banded leaf and sheath blight; pathogenesis-related protein; Rhizoctonia solani
KeyWords Plus: THAUMATIN-LIKE PROTEIN; TOBACCO; RESISTANCE; PLANTS; EXPRESSION; INFECTION; GENE; BETA-1,3-GLUCANASE; CHITINASE
通讯作者地址: Pan, GT (通讯作者),Sichuan Agr Univ, Maize Res Inst, Minist Agr, Key Lab Biol & Genet Improvement Maize SW China, Yaan 625014, Peoples R China
地址:
1. Sichuan Agr Univ, Maize Res Inst, Minist Agr, Key Lab Biol & Genet Improvement Maize SW China, Yaan 625014, Peoples R China
2. Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Sichuan, Peoples R China
电子邮件地址:
[email protected]基金资助致谢:
基金资助机构 授权号
Natural Science Foundation 30900901
Science and Technology Department Application Foundation of Sichuan Province 2006J13-039
Agriculture Project of Ministry 2008ZX08003-003
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This research was financially supported by the Natural Science Foundation (30900901), the Science and Technology Department Application Foundation of Sichuan Province (2006J13-039), and the Agriculture Project of Ministry (2008ZX08003-003). We thank Dr. Jihong Liu Clarke, who provided constructive criticism and helped improve the manuscript.
出版商: ACADEMIC JOURNALS, P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA
Web of Science 分类: Biotechnology & Applied Microbiology
学科类别: Biotechnology & Applied Microbiology
IDS 号: 868YD
ISSN: 1684-5315
SCI收录号：000298561500005
IDS 号: 868YD，这个数字在前，字母在后，表示是SCI收录，反过来就是ISTP收录
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