那位大侠帮着翻译一下啊？不要机器翻译的

Question

HEK293 cells were seeded onto 12-well plates and transfected with 0.5 μg of the Klotho promoter construct using Lipofectamine™
2000 (Invitrogen) according to the manufacturer's instructions. To
monitor transfection efficiency, 50 ng of the pRL-null plasmidencoding Renilla luciferase was included in all samples. Whereindicated, 0.2 μg mammalian expression vectors were also included. EGF induces Klotho mRNA expression. HEK293 cells were stimulated with 100 ng/mL EGF for different time periods. Total RNA was isolated and assessed for Klotho mRNA expression by Northern blot analysis (A) and relative expression levels of
Klotho mRNA were measured by quantitative real-time PCR (B). Relative levels were normalized to the level of gapdh mRNA. The data shown represent the mean±S.D. of three independent experiments performed in triplicate.

Answer 1

在被播种到12-well分别以0.5μg板和transfected的Klotho使用Lipofectamine™子构建
2000年(Invitrogen)按照制造商的说明。,
监控转染效率、50 ng的pRL-null plasmidencoding Renilla luciferase被包括在所有的样品。Whereindicated 0.2μg哺乳动物的表现也包含了进去。向量。Klotho mRNA表达诱使感受。分别受到刺激与100 ng / mL EGF为不同的时间。中分离出RNA和评估总数为Klotho表达mRNA北部污点(A)和相关分析结果的有效性
mRNA定量测定Klotho实时PCR(B)。相对含量水平gapdh归mRNA。数据显示代表平均值±s.d的三个独立的试验,一式三份。