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为了准确对玉米中还原糖和总糖进行定量分析,本论文对糖的测定方法进行了研究。在不同的参数下测定溶液的吸光度。如最佳测定波长、显色剂用量、反应时间、稳定时间、酸水解时间。本文实验采用DNS法,以3,5-二硝基水杨酸(DNS)为显色剂,在中性或碱性条件下与还原糖共热后被还原成棕红色的氨基化合物,其颜色深浅与糖含量呈正相关,通过单因素实验确定最佳实验条件,根据由葡萄糖标准溶液绘制的标准曲线计算玉米中还原糖和总糖含量。
实验结果表明,最佳检测波长为540nm,显色剂用量1.5mL,显色时间5min。葡萄糖的线性浓度范围为8~40ug/mL,以总糖溶液进行5次平行测定,RSD=1.1%;样品平均回收率为100.56%,RSD=1.54%。说明该方法稳定,重复性好。并通过对制得的样品溶液红外扫描谱图分析证明了溶液中含有还原糖。
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To return to original state accurately to the corn in the sugar and the total sugar carries on the quantitative analysis, the present paper has conducted the research to the sugar determination method. Determines the solution under the different parameter the extinction. Like best determination wave length, developer amount used, reaction time, settling time, acid hydrolysis time. This article tests uses the DNS law, take 3,5- dinitro naphthol salicylic acids (DNS) as the developer, in the neutrality or under the alkalinity condition heats after the return to original state sugar by the reduction brown red amino-compound, its color depth and the sugar content present are related, through the single factor experiment determined that the best experimental condition, according to returns to original state the sugar by in the glucose standard solution plan's specification curve computation corn and the total sugar content. the experimental result indicated that examines the wave length is best 540nm, developer amount used 1.5mL, colored time 5min. The glucose linear density scope is 8~40ug/mL, carries on 5 parallel determinations by the total sugar solution, RSD=1.1%; The sample mean returns-ratio is 100.56%, RSD=1.54%. Explained that this method is stable, the duplication is good. And through the sample solution infrared scanning spectrogram analysis which resulted to the system proves in the solution to include the return to original state sugar
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第1个回答  2011-05-31
In order to accurately to corn reducing sugar and total sugar, this paper quantitatively analyzed the determination method of sugar was studied. In different parameters were determined under the absorbance of the solution. Like the best measuring wavelength, colour-display reagent consumption, reaction time, stabilization time, acid hydrolysis time. The experiment using DNS law to 5-bis nitro salicylic acid (DNS) for colour-display reagent, in a neutral or alkaline conditions and reducing heat by reductive into of the palm red color depth amine-group compounds, which was positively associated with the sugar content by single factor experiment to determine the optimal experiment condition, according to standard solution by glucose drawing the standard curve calculation corn reducing sugars and total sugar content.

Experimental results show that the best 540nm detected wavelength for colour-display reagent consumption, 1.5 mL, show color time 5min. Glucose concentration range for the linear 40ug/mL, 8 ~ with total sugar solution 5 times parallel determination, RSD = 1.1%; The average recovery of 100.56% samples, RSD = 1.54%. That this method stability, excellent repeatability. And through the system of infrared scanning spectra sample solution proved solution contains analysis of reducing sugar.